Human HIF1a (Hypoxia Inducible Factor 1 Alpha) ELISA Kit Packaging:48 wells coli expression system and the

coli expression system and the target gene encoding Ser101-Lys198 is expressed

Recombinant Human Interleukin-13 is produced by our Mammalian expression system and the target gene encoding Gly21 ­ Asn132 is expressed with a Fc tag at the C-terminus

and the Human MPO standard plate wells that pre-coated using protein-related techniques are provided separately

The concentration of Universal HIS in tested samples can be calculated by comparing the OD of the samples to the standard curve

Human HIF1a (Hypoxia Inducible Factor 1 Alpha) ELISA Kit Packaging:48 wells coli expression system and theThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human HIF1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human HIF1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added,