Estradiol ELISA Kit Packaging:96 wells The OD value is proportional

The OD value is proportional to the concentration of Mouse TNFRSF1A

Then a biotinylated detection antibody specific for Human SEPP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

and the color intensity is directly proportional to the amount of Human BCAM present

Then a biotinylated detection antibody specific for Human TFPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Estradiol ELISA Kit Packaging:96 wells The OD value is proportionalThe Estradiol (E2) ELISA kit is based on the principle of delayed competitive binding assay between E2 in the test specimen and E2 enzyme conjugate for a constant amount of anti Estradiol monoclonal antibody epitope (Biotin reagent). In the incubation, anti E2 antibody biotin reagent, E2 standards, controls, and samples are incubated for 45 minutes at room temperature (RT), then E2 enzyme conjugate is added on the top of the reaction mixture and