Bovine AP (Apelin) ELISA Kit Packaging:96 wells a horseradish peroxidase conjugated mouse

a horseradish peroxidase conjugated mouse anti-Human TRAIL Antibody is added to the wells and binds to TRAIL in the sample

only those wells that contain Rat BRAK

The concentration of Human VEGF145 in the samples is then determined by comparing the OD of the samples to the standard curve

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BNP

Bovine AP (Apelin) ELISA Kit Packaging:96 wells a horseradish peroxidase conjugated mouseThe Bovine (AP) Apelin ELISA Kit measures Apelin in Bovine samples. The plate has been pre coated with Bovine APLN antibody. APLN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine APLN Antibody is added and binds to APLN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated APLN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.