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Chicken Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific to Pg
The plate has been pre-coated with Mouse NRG4 antibody
DISC1 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ELN
Human NINJ1 (Ninjurin-1) ELISA Kit PGFL Chicken Pg in samples (orThe Human (NINJ1) Ninjurin 1 ELISA Kit measures Ninjurin 1 in samples. The plate has been pre coated with Human NINJ1 antibody. NINJ1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human NINJ1 Antibody is added and binds to NINJ1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NINJ1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
