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Then a biotinylated detection antibody specific for Human IL-18 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
only those wells that contain Mouse IFN Gamma
The assay can be completed within 1 to 5 hours
The concentration of Rat TNNI3 in the samples is then determined by comparing the OD of the samples to the standard curve
QuicKey Pro Canine Progesterone ELISA Kit Packaging:48 wells Then a biotinylated detection antibodyThe QuicKey Pro Canine Progesterone ELISA kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Canine Pg. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine Pg are added to the micro ELISA plate wells. Canine Pg in samples (or standards) competes with a fixed amount of Pg on the solid phase supporter for sites on the HRP linked detection antibody specific
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