Human CRYAB (Alpha-crystallin B chain) ELISA Kit OS3 Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Rat PRL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

AQP7 present in the sample is added and binds to antibodies coated on the wells

Substrate solution is then added and color develops in proportion to the amount of Rat CCL7

Then Streptavidin-HRP is added and binds to the Biotinylated CLEC5A antibody

Human CRYAB (Alpha-crystallin B chain) ELISA Kit OS3 Then a biotinylated detection antibodyThe Human CRYAB (Alpha crystallin B chain) ELISA Kit measures CRYAB in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CRYAB antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated