Human GPC4 (Glypican 4) ELISA Kit Beta-Urogastrone Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KRT18

The Canine (FN) Fibronectin ELISA Kit measures Fibronectin in Canine samples

Substrate solution is then added and color develops in proportion to the amount of Human AOC3

Then Streptavidin-HRP is added and binds to the Biotinylated HBB antibody

Human GPC4 (Glypican 4) ELISA Kit Beta-Urogastrone Standards or samples are addedThe Human (GPC4) Glypican 4 ELISA Kit measures Glypican 4 in samples. The plate has been pre coated with Human GPC4 antibody. GPC4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human GPC4 Antibody is added and binds to GPC4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GPC4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.