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The microtiter plate is coated with the antibody specifically binding Insulin-like Growth Factor-binding Protein 7
Only those wells that contain Human SP-D
Only those wells that contain Human RANκL
The concentration of Guinea pig IL4 in the samples is then determined by comparing the OD of the samples to the standard curve
GDF1 (Growth Differentiation Factor 1) ELISA Kit (Small Sample) Packaging:48 wells The microtiter plate is coatedThe GDF1 (Growth Differentiation Factor 1) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human GDF1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human GDF1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
