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Substrate solution is then added and color develops in proportion to the amount of Rat KRT15
Substrate solution is then added and color develops in proportion to the amount of Human BDKRB1
And then biotinylated Rat HMGB1 Antibody is added and binds to HMGB1 in the sample
Then Streptavidin-HRP is added and binds to the Biotinylated CFP antibody
Human TPO (Thyroid-Peroxidase) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (TPO) Thyroid Peroxidase ELISA Kit measures Thyroid Peroxidase in samples. The plate has been pre coated with Human TPO antibody. TPO present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TPO Antibody is added and binds to TPO in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TPO antibody. After incubation unbound Streptavidin HRP is washed away during a washing
