Rat FN (Fibronectin) ELISA Kit PRO2275 Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DEFa3

The concentration of Human MUC13 in the samples is then determined by comparing the OD of the samples to the standard curve

And then biotinylated Mouse RSPO4 Antibody is added and binds to RSPO4 in the sample

The Human Vitamin D Receptor ELISA kit uses the Sandwich-ELISA principle

Rat FN (Fibronectin) ELISA Kit PRO2275 Standards or samples are addedThe Rat (FN) Fibronectin ELISA Kit measures Fibronectin in Rat samples. The plate has been pre coated with Rat FN antibody. FN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat FN Antibody is added and binds to FN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated FN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution