Mouse CASP9 (Caspase 9) ELISA Kit Packaging:96 wells Substrate solution is then added

Substrate solution is then added and color develops in proportion to the amount of Human YWHAB

And then biotinylated Rabbit ACE Antibody is added and binds to ACE in the sample

only those wells that contain Mouse LPAR3

After incubation unbound Streptavidin- HRP is washed away during a washing step

Mouse CASP9 (Caspase 9) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Mouse CASP9 (Caspase 9) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse CASP9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse CASP9 and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well