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Then a biotinylated detection antibody specific for Mouse IgG1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
only those wells that contain Human PDCD4
Then Streptavidin-HRP is added and binds to the Biotinylated JTB antibody
And then biotinylated Mouse CUBN Antibody is added and binds to CUBN in the sample
Human LCN15 (Lipocalin 15) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human (LCN15) Lipocalin 15 ELISA Kit measures Lipocalin 15 in samples. The plate has been pre coated with Human LCN15 antibody. LCN15 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LCN15 Antibody is added and binds to LCN15 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LCN15 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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