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Human MT in the sample or standard competes with a fixed amount of Human MT on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human MT
The OD value is proportional to the concentration of Human CD74
The concentration of Human TPS in the samples is then determined by comparing the OD of the samples to the standard curve
You can calculate the concentration of Human IL-1Beta in the samples by comparing the OD of the samples to the standard curve
Human SPARC ELISA Kit Lymphotoxin Alpha Human MT in the sampleThe Human SPARC ELISA Kit measures SPARC in samples. The plate has been pre coated with Human SPARC antibody. SPARC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SPARC Antibody is added and binds to SPARC in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SPARC antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
