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When the sample containing B7-H3 is added to the plate
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 32 nm
Then a biotinylated detection antibody specific for AGT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
and the color intensity is directly proportional to the amount of Human IL-4 present
Mouse GLI1 (Zinc Finger Protein Gli1) ELISA Kit Versican Proteoglycan When the sample containing B7-H3The Mouse (GLI1) Zinc Finger Protein Gli1 ELISA Kit measures Zinc Finger Protein Gli1 in Mouse samples. The plate has been pre coated with Mouse GLI1 antibody. GLI1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse GLI1 Antibody is added and binds to GLI1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GLI1 antibody. After incubation unbound Streptavidin HRP is washed
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