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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SIRT1
The plate has been pre-coated with Human CHST10 antibody
PPM1B present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated IRF5 antibody
Mouse anti-cell free DNA ELISA Kit Packaging:96 wells Standards or samples are addedThe Mouse anti cell free DNA ELISA Kit measures Anti cell free DNA in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on indirect ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with antigen. HRP antibody is used as detection antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP
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