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Then a biotinylated detection antibody specific for REN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 36 nm
The plate has been pre-coated with Human BRCC3 antibody
only those wells that contain Rat IL12
Human GSH (Glutathione) ELISA Kit THPH8 Then a biotinylated detection antibodyThe Human (GSH) Glutathione ELISA Kit measures glutathione in samples. Add samples to the pre coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin HRP is then added and then incubate. Unbound avidin HRP is washed away during a washing step. TMB Substrate is then added and color
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