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CRKL present in the sample is added and binds to antibodies coated on the wells
The concentration of LILRA2 in the sample was calculated by drawing a standard curve
Substrate solution is then added and color develops in proportion to the amount of Mouse EDA
Then a biotinylated detection antibody specific for Human PAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mouse PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit Packaging:96 wells CRKL present in the sampleThe Mouse PACAP (Pituitary Adenylate Cyclase Activating Polypeptide) ELISA Kit measures PACAP in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti PACAP antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound
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