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Then a biotinylated detection antibody specific for Human UCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
TFAM present in the sample is added and binds to antibodies coated on the wells
only those wells that contain Human CD24
The plate has been pre-coated with Human CHREBP antibody
Rat PON1 (Paraoxonase-1) ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Rat (PON1) Paraoxonase 1 ELISA Kit measures Paraoxonase 1 in Rat samples. The plate has been pre coated with Rat PON1 antibody. PON1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PON1 Antibody is added and binds to PON1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PON1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
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