Mouse OSM (Oncostatin-M) ELISA Kit ApoAIV Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ZP2

Substrate solution is then added and color develops in proportion to the amount of Mouse CISD2

The concentration of Mouse TTR in the samples is then determined by comparing the OD of the samples to the standard curve

Then a biotinylated detection antibody specific for Human NRG-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Mouse OSM (Oncostatin-M) ELISA Kit ApoAIV Standards or samples are addedThe Mouse (OSM) Oncostatin M ELISA Kit measures Oncostatin M in Mouse samples. The plate has been pre coated with Mouse OSM antibody. OSM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse OSM Antibody is added and binds to OSM in the sample. Then Streptavidin HRP is added and binds to the Biotinylated OSM antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.