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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ECM1
and the absorbance is spectrophotometrically measured at 450nm
The OD value is proportional to the concentration of Rat CT
The concentration of Mouse PTHrP in the samples is then determined by comparing the OD of the samples to the standard curve
Rat SST (Somatostatin) ELISA Kit (Small Sample) HLP Standards or samples are addedThe Rat SST (Somatostatin) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Rat SST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Rat SST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only
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