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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle PTN
Then Streptavidin-HRP is added and binds to the Biotinylated CORO2A antibody
And then biotinylated Human BCAT2 Antibody is added and binds to BCAT2 in the sample
The assay plate has been pre-coated with mouse anti-Human JAM-A monoclonal antibody
Human CFL2 (Cofilin-2) ELISA Kit HVEML Standards or samples are addedThe Human (CFL2) Cofilin 2 ELISA Kit measures Cofilin 2 in samples. The plate has been pre coated with Human CFL2 antibody. CFL2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CFL2 Antibody is added and binds to CFL2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CFL2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
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