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Then a biotinylated detection antibody specific for Mouse NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Mouse HRH4 in the samples is then determined by comparing the OD of the samples to the standard curve
Then Streptavidin-HRP is added and binds to the Biotinylated CST5 antibody
The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of RBP4 in the specimen
Rat SMAD5 (Mothers Against Decapentaplegic Homolog 5) ELISA Kit HPSE1 Then a biotinylated detection antibodyThe Rat (SMAD5) Mothers Against Decapentaplegic Homolog 5 ELISA Kit measures Mothers Against Decapentaplegic Homolog 5 in Rat samples. The plate has been pre coated with Rat Smad5 antibody. Smad5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat Smad5 Antibody is added and binds to Smad5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated Smad5 antibody. After incubation
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