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EN2 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IL17C
Then Streptavidin-HRP is added and binds to the Biotinylated MAP2K4 antibody
The OD value is proportional to the concentration of Mouse GRN
Human U2 (Urotensin 2) ELISA Kit Packaging:96 wells EN2 present in the sampleThe Human (U2) Urotensin 2 ELISA Kit measures Urotensin 2 in samples. The plate has been pre coated with Human U2 antibody. U2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human U2 Antibody is added and binds to U2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated U2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
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