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The concentration of Rat LBP in the samples is then determined by comparing the OD of the samples to the standard curve
GOT1 present in the sample is added and binds to antibodies coated on the wells
Then a biotinylated detection antibody specific for Rat FAS/CD95 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig TGFb1
Human TCFL5 (Transcription Factor-like 5 Protein) ELISA Kit Packaging:96 wells The concentration of Rat LBPThe Human (TCFL5) Transcription Factor like 5 Protein ELISA Kit measures Transcription factor like 5 protein in samples. The plate has been pre coated with Human TCFL5 antibody. TCFL5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human TCFL5 Antibody is added and binds to TCFL5 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated TCFL5 antibody. After incubation unbound
