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Substrate solution is then added and color develops in proportion to the amount of Human SPARC
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL22Ra2
FGF23 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated UPK3A antibody
Human WNT5B (Protein Wnt-5B) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (WNT5B) Protein Wnt 5B ELISA Kit measures Protein Wnt 5B in samples. The plate has been pre coated with Human WNT5B antibody. WNT5B present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human WNT5B Antibody is added and binds to WNT5B in the sample. Then Streptavidin HRP is added and binds to the Biotinylated WNT5B antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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