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The assay plate has been pre-coated with mouse anti-Human Cystatin C monoclonal antibody
such that spontaneous hydrolysis will not occur for many minutes
Then a biotinylated detection antibody specific for Human MMP-14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human IgG in the samples is then determined by comparing the OD of the samples to the standard curve
Mouse a1ACT (Alpha-1-Antichymotrypsin) ELISA Kit (Small Sample) Packaging:96 wells The assay plate has beenThe Mouse a1ACT (Alpha 1 Antichymotrypsin) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse a1ACT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse a1ACT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate
