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TMB substrates were added to visualize HRP enzymatic reaction
a horseradish peroxidase conjugated mouse anti-Ferret TNF-alpha Antibody is added to the wells and binds to TNF- alpha in the sample
BMP6 present in the sample is added and binds to antibodies coated on the wells
Substrate solution is then added and color develops in proportion to the amount of Human CCT6A
Human DNM1L (Dynamin-1-like protein) ELISA Kit Packaging:96 wells TMB substrates were added toThe Human DNM1L (Dynamin 1 like protein) ELISA Kit measures DNM1L in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti DNM1L antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated
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