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Positive/Negative Controls or samples are added to the wells and incubate
The concentration of Human hs-Hepc25 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GDF2
and the absorbance is measured spectrophotometrically at 450nm
Human CAT (Catalase) ELISA Kit Packaging:96 wells Positive/Negative Controls or samples areThe Human (CAT) Catalase ELISA Kit measures Catalase in samples. The plate has been pre coated with Human CAT antibody. CAT present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CAT Antibody is added and binds to CAT in the sample. Then Streptavidin HRP is added and binds to the Biotinylated CAT antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
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