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The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle PLGF
then added a HRP-conjugated antibody specific to Human GH
The concentration of Mouse MDGA1 in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat C4
Rat AP (Apelin) ELISA Kit Packaging:96 wells The microtiter plate provided inThe Rat (AP) Apelin ELISA Kit measures Apelin in Rat samples. The plate has been pre coated with Rat APLN antibody. APLN present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat APLN Antibody is added and binds to APLN in the sample. Then Streptavidin HRP is added and binds to the Biotinylated APLN antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution
