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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit PF4
The concentration of Chicken IL5 in the samples is then determined by comparing the OD of the samples to the standard curve
Only those wells that contain Mouse GM-CSF
The OD value is proportional to the concentration of Rat COL3
Leptin ELISA Kit UP-1 Standards or samples are addedThe Leptin ELISA kit is a two step capture or sandwich type immunoassay to measure Leptin in serum. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for leptin is immobilized onto the microplate and another monoclonal antibody specific for a different epitope of leptin is conjugated to biotin (biotin conjugate). In the first incubation step, leptin present in the specimen samples, calibrators and
