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Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue color
The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm
Only those wells that contain Mouse ALPL
The OD value is proportional to the concentration of PF4
Human ABL2 (Tyrosine-protein Kinase Abl2) ELISA Kit Packaging:96 wells Horseradish peroxidase (HRP) bound inThe Human (ABL2) Tyrosine protein Kinase Abl2 ELISA Kit measures Tyrosine protein kinase ABL2 in samples. The plate has been pre coated with Human ABL2 antibody. ABL2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ABL2 Antibody is added and binds to ABL2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated ABL2 antibody. After incubation unbound Streptavidin HRP is washed
