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And then biotinylated Rat ROCK1 Antibody is added and binds to ROCK1 in the sample
And then biotinylated Human MOCOS Antibody is added and binds to MOCOS in the sample
Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of T in the sample
Calculate the concentration of AKR1C1 in the sample by plotting standard curve
Mouse CAN (Calcineurin) ELISA Kit HEL168 And then biotinylated Rat ROCK1The Mouse CAN (Calcineurin) ELISA Kit measures CaN in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti CaN antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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