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Substrate solution is then added and color develops in proportion to the amount of Human CAT
The plate has been pre-coated with Human FMR1NB antibody
Then a biotinylated detection antibody specific for Mouse Pro-MMP-9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Human TNFSF4 in the samples is then determined by comparing the OD of the samples to the standard curve
Human MUC2 (Mucin-2) ELISA Kit IL-12/p70 Substrate solution is then addedThe Human (MUC2) Mucin 2 ELISA Kit measures Mucin 2 in samples. The plate has been pre coated with Human MUC2 antibody. MUC2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human MUC2 Antibody is added and binds to MUC2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated MUC2 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
