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This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10
MRC1 present in the sample is added and binds to antibodies coated on the wells
Then Streptavidin-HRP is added and binds to the Biotinylated PLet1 antibody
The OD value is proportional to the concentration of Rat CGRP1
Rat PRDX1 (Peroxiredoxin 1) ELISA Kit Packaging:96 wells This conjugation reaction is mediatedThe Rat (PRDX1) Peroxiredoxin 1 ELISA Kit measures Peroxiredoxin 1 in Rat samples. The plate has been pre coated with Rat PRDX1 antibody. PRDX1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat PRDX1 Antibody is added and binds to PRDX1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PRDX1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
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