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only those wells that contain Human PFN1
Then a biotinylated detection antibody specific for Mouse NT-proBNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
The concentration of Mouse PIINP in the samples is then determined by comparing the OD of the samples to the standard curve
Human FE in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human FE
Mouse LY6D (Lymphocyte Antigen 6D) ELISA Kit Gp39 only those wells that containThe Mouse (LY6D) Lymphocyte Antigen 6D ELISA Kit measures Lymphocyte antigen 6D in Mouse samples. The plate has been pre coated with Mouse LY6D antibody. LY6D present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Mouse LY6D Antibody is added and binds to LY6D in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LY6D antibody. After incubation unbound Streptavidin HRP is washed away
