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CCT8 present in the sample is added and binds to antibodies coated on the wells
The concentration of Human KRT17 in the samples is then determined by comparing the OD of the samples to the standard curve
The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 18 nm
In the incubation
Rat HMGB-1 (High Mobility Group Protein B1) ELISA Kit Packaging:96 wells CCT8 present in the sampleThe Rat (HMGB 1) High Mobility Group Protein B1 ELISA Kit measures High Mobility Group Protein B1 in Rat samples. The plate has been pre coated with Rat HMGB1 antibody. HMGB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat HMGB1 Antibody is added and binds to HMGB1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated HMGB1 antibody. After incubation unbound Streptavidin HRP
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