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Substrate solution is then added and color develops in proportion to the amount of Human JAM3
The OD value is proportional to the concentration of Human PTX3/TSG-14
You can calculate the concentration of Human sTREM-1 in the samples by comparing the OD of the samples to the standard curve
plasma or BALF is incubated in the plate with the capture antibody
Human RGS2 (Regulator of G-protein Signaling 2) ELISA Kit Packaging:96 wells Substrate solution is then addedThe Human (RGS2) Regulator of G protein Signaling 2 ELISA Kit measures Regulator of G protein signaling 2 in samples. The plate has been pre coated with Human RGS2 antibody. RGS2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human RGS2 Antibody is added and binds to RGS2 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated RGS2 antibody. After incubation unbound Streptavidin