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Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour
The concentration of Human CER in the samples is then determined by comparing the OD of the samples to the standard curve
only those wells that contain Human LTF and HRP-conjugated antibody will exhibit a change in color
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse bTG
NPTX1 (Neuronal Pentraxin I) ELISA Kit (Small Sample) IGFBP8 Horseradish peroxidase (HRP) bound inThe NPTX1 (Neuronal Pentraxin I) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Human NPTX1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Human NPTX1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
