Rat GLO1 (Lactoylglutathione Lyase) ELISA Kit IL-1RP1 Standards or samples are added

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to ECF

And then biotinylated Rat KRT15 Antibody is added and binds to KRT15 in the sample

SIGLEC1 present in the sample is added and binds to antibodies coated on the wells

Then a biotinylated detection antibody specific for Human EGFR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Rat GLO1 (Lactoylglutathione Lyase) ELISA Kit IL-1RP1 Standards or samples are addedThe Rat (GLO1) Lactoylglutathione Lyase ELISA Kit measures Lactoylglutathione Lyase in Rat samples. The plate has been pre coated with Rat GLO1 antibody. GLO1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat GLO1 Antibody is added and binds to GLO1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated GLO1 antibody. After incubation unbound Streptavidin HRP is washed away