Human KIRREL (Kin of IRRE-like protein 1) ELISA Kit Packaging:96 wells The concentration of Rat IL21

The concentration of Rat IL21 in the samples is then determined by comparing the OD of the samples to the standard curve

Then Streptavidin-HRP is added and binds to the Biotinylated CFB antibody

Then a biotinylated detection antibody specific for Mouse VCAM-1/CD106 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Then a biotinylated detection antibody specific for Human PCT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

Human KIRREL (Kin of IRRE-like protein 1) ELISA Kit Packaging:96 wells The concentration of Rat IL21The Human KIRREL (Kin of IRRE like protein 1) ELISA Kit measures KIRREL in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti KIRREL antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated